Gene Expression:Article Title: Aspirin alleviates pulmonary fibrosis through PI3K/AKT/mTOR-mediated autophagy pathway.
Article Snippet: Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were obtained from The Global Bioresource Center (ATCC® CCL-134TM, United States).. Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.
Article Title: Inhibition of Sirt2 Alleviates Fibroblasts Activation and Pulmonary Fibrosis via Smad2/3 Pathway
Article Snippet: The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were purchased from The Global Bioresource Center (ATCC® CCL-134TM, United States).. In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells were cultured for a period of 0, 3, 6, 12, 24, and 48 h at 2 ng/ml TGF- β 1.In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells..
Transfection:Article Title: Aspirin alleviates pulmonary fibrosis through PI3K/AKT/mTOR-mediated autophagy pathway.
Article Snippet: Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were obtained from The Global Bioresource Center (ATCC® CCL-134TM, United States).. Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.
Article Title: Inhibition of Sirt2 Alleviates Fibroblasts Activation and Pulmonary Fibrosis via Smad2/3 Pathway
Article Snippet: The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were purchased from The Global Bioresource Center (ATCC® CCL-134TM, United States).. In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells were cultured for a period of 0, 3, 6, 12, 24, and 48 h at 2 ng/ml TGF- β 1.In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells..
Expressing:Article Title: Aspirin alleviates pulmonary fibrosis through PI3K/AKT/mTOR-mediated autophagy pathway.
Article Snippet: Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were obtained from The Global Bioresource Center (ATCC® CCL-134TM, United States).. Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.
Article Title: Inhibition of Sirt2 Alleviates Fibroblasts Activation and Pulmonary Fibrosis via Smad2/3 Pathway
Article Snippet: The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were purchased from The Global Bioresource Center (ATCC® CCL-134TM, United States).. In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells were cultured for a period of 0, 3, 6, 12, 24, and 48 h at 2 ng/ml TGF- β 1.In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells..
Western Blot:Article Title: Aspirin alleviates pulmonary fibrosis through PI3K/AKT/mTOR-mediated autophagy pathway.
Article Snippet: Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were obtained from The Global Bioresource Center (ATCC® CCL-134TM, United States).. Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.
Article Title: Inhibition of Sirt2 Alleviates Fibroblasts Activation and Pulmonary Fibrosis via Smad2/3 Pathway
Article Snippet: The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were purchased from The Global Bioresource Center (ATCC® CCL-134TM, United States).. In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells were cultured for a period of 0, 3, 6, 12, 24, and 48 h at 2 ng/ml TGF- β 1.In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells..
Quantitative RT-PCR:Article Title: Aspirin alleviates pulmonary fibrosis through PI3K/AKT/mTOR-mediated autophagy pathway.
Article Snippet: Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were obtained from The Global Bioresource Center (ATCC® CCL-134TM, United States).. Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.
Article Title: Inhibition of Sirt2 Alleviates Fibroblasts Activation and Pulmonary Fibrosis via Smad2/3 Pathway
Article Snippet: The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were purchased from The Global Bioresource Center (ATCC® CCL-134TM, United States).. In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells were cultured for a period of 0, 3, 6, 12, 24, and 48 h at 2 ng/ml TGF- β 1.In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells..
Control:Article Title: Aspirin alleviates pulmonary fibrosis through PI3K/AKT/mTOR-mediated autophagy pathway.
Article Snippet: Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were obtained from The Global Bioresource Center (ATCC® CCL-134TM, United States).. Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.
Article Title: Inhibition of Sirt2 Alleviates Fibroblasts Activation and Pulmonary Fibrosis via Smad2/3 Pathway
Article Snippet: The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were purchased from The Global Bioresource Center (ATCC® CCL-134TM, United States).. In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells were cultured for a period of 0, 3, 6, 12, 24, and 48 h at 2 ng/ml TGF- β 1.In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells..
Negative Control:Article Title: Aspirin alleviates pulmonary fibrosis through PI3K/AKT/mTOR-mediated autophagy pathway.
Article Snippet: Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were obtained from The Global Bioresource Center (ATCC® CCL-134TM, United States).. Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.
Article Title: Inhibition of Sirt2 Alleviates Fibroblasts Activation and Pulmonary Fibrosis via Smad2/3 Pathway
Article Snippet: The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were purchased from The Global Bioresource Center (ATCC® CCL-134TM, United States).. In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells were cultured for a period of 0, 3, 6, 12, 24, and 48 h at 2 ng/ml TGF- β 1.In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells..
Small Interfering RNA:Article Title: Aspirin alleviates pulmonary fibrosis through PI3K/AKT/mTOR-mediated autophagy pathway.
Article Snippet: Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).Human embryonic lung fibroblasts (MRC-5) were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were obtained from The Global Bioresource Center (ATCC® CCL-134TM, United States).. Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.Cells were cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal bovine serum (FBS) (Gibco, United States), 100U/mL penicillin, and 100μg/mL streptomycin at 37 ◦C in 5 % CO2 atmosphere incubator.
Article Title: Inhibition of Sirt2 Alleviates Fibroblasts Activation and Pulmonary Fibrosis via Smad2/3 Pathway
Article Snippet: The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).The human embryonic lung fibroblasts (MRC-5) used in this study were purchased from the Chinese academy of sciences (Cat. no. GNHu41, Shanghai, China).. Human primary IPF lung fibroblasts were purchased from The Global Bioresource Center (ATCC® CCL-134TM, United States).. In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells were cultured for a period of 0, 3, 6, 12, 24, and 48 h at 2 ng/ml TGF- β 1.In TGF- β 1 concentration-dependent assay, when the MRC-5 reached 80% confluence, the growth medium was changed to serum free medium overnight; then the cells were treated with Recombinant human TGF- β 1 (R&D Systems, Minneapolis, MN) at 0, 1, 2, 5, and 10 ng/ml for 24 h. In the time-dependent test, the cells..
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